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smart-seq2 libraries  (Illumina Inc)


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    Structured Review

    Illumina Inc smart-seq2 libraries
    Smart Seq2 Libraries, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smart-seq2+libraries/smart+seq2+libraries/pmc11127180-700-0-6
    Average 90 stars, based on 1 article reviews
    smart-seq2 libraries - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: Tuft cell-produced cysteinyl leukotrienes and IL-25 synergistically initiate lung type 2 inflammation.
    Article Snippet: Smart-seq2 libraries were sequenced on an Illumina NextSeq 500 using a High Output kit to generate 2 × 38–base pair reads (plus dual index reads).

    Article Title: Depletion of Hemoglobin Transcripts and Long-Read Sequencing Improves the Transcriptome Annotation of the Polar Bear ( Ursus maritimus )
    Article Snippet: We then sequenced the Smart-seq2 libraries on a multiplexed Illumina HiSeq X 2 × 151 bp run.

    Article Title: Naive stem cell blastocyst model captures human embryo lineage segregation.
    Article Snippet: Smart-seq2 libraries were prepared as described (Picelli et al., 2013) and sequenced using the Illumina Novaseq platform in paired end format.

    Article Title: Generation of an isoform-level transcriptome atlas of macrophage activation
    Article Snippet: Bottom , we extracted RNA and synthesized full-length cDNA which we then processed to generate Smart-seq2 and R2C2 libraries for Illumina and Oxford Nanopore Technologies (ONT) sequencing, respectively.

    Article Title: The cysteinyl leukotriene 3 receptor regulates expansion of IL-25-producing airway brush cells leading to type 2 inflammation.
    Article Snippet: Briefly, Smart-seq2 libraries were sequenced on an Illumina NextSeq500 using a High Output kit to generate 2 × 25 bp reads (plus dual index reads).

    Article Title: Naive stem cell blastocyst model captures human embryo lineage segregation
    Article Snippet: Smart-seq2 libraries were prepared as described ( ) and sequenced using the Illumina Novaseq platform in paired end format.

    Article Title: Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis
    Article Snippet: Smart-seq2 libraries were prepared and sequenced using an Illumina NextSeq500.

    Article Title: A nasal cell atlas reveals heterogeneity of tuft cells and their role in directing olfactory stem cell proliferation
    Article Snippet: Smart-seq2 libraries were sequenced on an Illumina NextSeq500 using a High Output kit to generate 2 x 25 bp reads.

    Sequencing:

    Article Title: Tuft cell-produced cysteinyl leukotrienes and IL-25 synergistically initiate lung type 2 inflammation.
    Article Snippet: Smart-seq2 libraries were sequenced on an Illumina NextSeq 500 using a High Output kit to generate 2 × 38–base pair reads (plus dual index reads).

    Article Title: Depletion of Hemoglobin Transcripts and Long-Read Sequencing Improves the Transcriptome Annotation of the Polar Bear ( Ursus maritimus )
    Article Snippet: We then sequenced the Smart-seq2 libraries on a multiplexed Illumina HiSeq X 2 × 151 bp run.

    Article Title: Naive stem cell blastocyst model captures human embryo lineage segregation.
    Article Snippet: Smart-seq2 libraries were prepared as described (Picelli et al., 2013) and sequenced using the Illumina Novaseq platform in paired end format.

    Article Title: Generation of an isoform-level transcriptome atlas of macrophage activation
    Article Snippet: Bottom , we extracted RNA and synthesized full-length cDNA which we then processed to generate Smart-seq2 and R2C2 libraries for Illumina and Oxford Nanopore Technologies (ONT) sequencing, respectively.

    Article Title: The cysteinyl leukotriene 3 receptor regulates expansion of IL-25-producing airway brush cells leading to type 2 inflammation.
    Article Snippet: Briefly, Smart-seq2 libraries were sequenced on an Illumina NextSeq500 using a High Output kit to generate 2 × 25 bp reads (plus dual index reads).

    Article Title: Naive stem cell blastocyst model captures human embryo lineage segregation
    Article Snippet: Smart-seq2 libraries were prepared as described ( ) and sequenced using the Illumina Novaseq platform in paired end format.

    Article Title: Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis
    Article Snippet: Smart-seq2 libraries were prepared and sequenced using an Illumina NextSeq500.

    Article Title: A nasal cell atlas reveals heterogeneity of tuft cells and their role in directing olfactory stem cell proliferation
    Article Snippet: Smart-seq2 libraries were sequenced on an Illumina NextSeq500 using a High Output kit to generate 2 x 25 bp reads.

    Activation Assay:

    Article Title: Tuft cell-produced cysteinyl leukotrienes and IL-25 synergistically initiate lung type 2 inflammation.
    Article Snippet: Smart-seq2 libraries were sequenced on an Illumina NextSeq 500 using a High Output kit to generate 2 × 38–base pair reads (plus dual index reads).

    Article Title: Depletion of Hemoglobin Transcripts and Long-Read Sequencing Improves the Transcriptome Annotation of the Polar Bear ( Ursus maritimus )
    Article Snippet: We then sequenced the Smart-seq2 libraries on a multiplexed Illumina HiSeq X 2 × 151 bp run.

    Article Title: Naive stem cell blastocyst model captures human embryo lineage segregation.
    Article Snippet: Smart-seq2 libraries were prepared as described (Picelli et al., 2013) and sequenced using the Illumina Novaseq platform in paired end format.

    Article Title: Generation of an isoform-level transcriptome atlas of macrophage activation
    Article Snippet: Bottom , we extracted RNA and synthesized full-length cDNA which we then processed to generate Smart-seq2 and R2C2 libraries for Illumina and Oxford Nanopore Technologies (ONT) sequencing, respectively.

    Article Title: The cysteinyl leukotriene 3 receptor regulates expansion of IL-25-producing airway brush cells leading to type 2 inflammation.
    Article Snippet: Briefly, Smart-seq2 libraries were sequenced on an Illumina NextSeq500 using a High Output kit to generate 2 × 25 bp reads (plus dual index reads).

    Article Title: Naive stem cell blastocyst model captures human embryo lineage segregation
    Article Snippet: Smart-seq2 libraries were prepared as described ( ) and sequenced using the Illumina Novaseq platform in paired end format.

    Article Title: Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis
    Article Snippet: Smart-seq2 libraries were prepared and sequenced using an Illumina NextSeq500.

    Article Title: A nasal cell atlas reveals heterogeneity of tuft cells and their role in directing olfactory stem cell proliferation
    Article Snippet: Smart-seq2 libraries were sequenced on an Illumina NextSeq500 using a High Output kit to generate 2 x 25 bp reads.

    Generated:

    Article Title: Tuft cell-produced cysteinyl leukotrienes and IL-25 synergistically initiate lung type 2 inflammation.
    Article Snippet: Smart-seq2 libraries were sequenced on an Illumina NextSeq 500 using a High Output kit to generate 2 × 38–base pair reads (plus dual index reads).

    Article Title: Depletion of Hemoglobin Transcripts and Long-Read Sequencing Improves the Transcriptome Annotation of the Polar Bear ( Ursus maritimus )
    Article Snippet: We then sequenced the Smart-seq2 libraries on a multiplexed Illumina HiSeq X 2 × 151 bp run.

    Article Title: Naive stem cell blastocyst model captures human embryo lineage segregation.
    Article Snippet: Smart-seq2 libraries were prepared as described (Picelli et al., 2013) and sequenced using the Illumina Novaseq platform in paired end format.

    Article Title: Generation of an isoform-level transcriptome atlas of macrophage activation
    Article Snippet: Bottom , we extracted RNA and synthesized full-length cDNA which we then processed to generate Smart-seq2 and R2C2 libraries for Illumina and Oxford Nanopore Technologies (ONT) sequencing, respectively.

    Article Title: The cysteinyl leukotriene 3 receptor regulates expansion of IL-25-producing airway brush cells leading to type 2 inflammation.
    Article Snippet: Briefly, Smart-seq2 libraries were sequenced on an Illumina NextSeq500 using a High Output kit to generate 2 × 25 bp reads (plus dual index reads).

    Article Title: Naive stem cell blastocyst model captures human embryo lineage segregation
    Article Snippet: Smart-seq2 libraries were prepared as described ( ) and sequenced using the Illumina Novaseq platform in paired end format.

    Article Title: Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis
    Article Snippet: Smart-seq2 libraries were prepared and sequenced using an Illumina NextSeq500.

    Article Title: A nasal cell atlas reveals heterogeneity of tuft cells and their role in directing olfactory stem cell proliferation
    Article Snippet: Smart-seq2 libraries were sequenced on an Illumina NextSeq500 using a High Output kit to generate 2 x 25 bp reads.

    Derivative Assay:

    Article Title: Tuft cell-produced cysteinyl leukotrienes and IL-25 synergistically initiate lung type 2 inflammation.
    Article Snippet: Smart-seq2 libraries were sequenced on an Illumina NextSeq 500 using a High Output kit to generate 2 × 38–base pair reads (plus dual index reads).

    Article Title: Depletion of Hemoglobin Transcripts and Long-Read Sequencing Improves the Transcriptome Annotation of the Polar Bear ( Ursus maritimus )
    Article Snippet: We then sequenced the Smart-seq2 libraries on a multiplexed Illumina HiSeq X 2 × 151 bp run.

    Article Title: Naive stem cell blastocyst model captures human embryo lineage segregation.
    Article Snippet: Smart-seq2 libraries were prepared as described (Picelli et al., 2013) and sequenced using the Illumina Novaseq platform in paired end format.

    Article Title: Generation of an isoform-level transcriptome atlas of macrophage activation
    Article Snippet: Bottom , we extracted RNA and synthesized full-length cDNA which we then processed to generate Smart-seq2 and R2C2 libraries for Illumina and Oxford Nanopore Technologies (ONT) sequencing, respectively.

    Article Title: The cysteinyl leukotriene 3 receptor regulates expansion of IL-25-producing airway brush cells leading to type 2 inflammation.
    Article Snippet: Briefly, Smart-seq2 libraries were sequenced on an Illumina NextSeq500 using a High Output kit to generate 2 × 25 bp reads (plus dual index reads).

    Article Title: Naive stem cell blastocyst model captures human embryo lineage segregation
    Article Snippet: Smart-seq2 libraries were prepared as described ( ) and sequenced using the Illumina Novaseq platform in paired end format.

    Article Title: Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis
    Article Snippet: Smart-seq2 libraries were prepared and sequenced using an Illumina NextSeq500.

    Article Title: A nasal cell atlas reveals heterogeneity of tuft cells and their role in directing olfactory stem cell proliferation
    Article Snippet: Smart-seq2 libraries were sequenced on an Illumina NextSeq500 using a High Output kit to generate 2 x 25 bp reads.

    Expressing:

    Article Title: Tuft cell-produced cysteinyl leukotrienes and IL-25 synergistically initiate lung type 2 inflammation.
    Article Snippet: Smart-seq2 libraries were sequenced on an Illumina NextSeq 500 using a High Output kit to generate 2 × 38–base pair reads (plus dual index reads).

    Article Title: Depletion of Hemoglobin Transcripts and Long-Read Sequencing Improves the Transcriptome Annotation of the Polar Bear ( Ursus maritimus )
    Article Snippet: We then sequenced the Smart-seq2 libraries on a multiplexed Illumina HiSeq X 2 × 151 bp run.

    Article Title: Naive stem cell blastocyst model captures human embryo lineage segregation.
    Article Snippet: Smart-seq2 libraries were prepared as described (Picelli et al., 2013) and sequenced using the Illumina Novaseq platform in paired end format.

    Article Title: Generation of an isoform-level transcriptome atlas of macrophage activation
    Article Snippet: Bottom , we extracted RNA and synthesized full-length cDNA which we then processed to generate Smart-seq2 and R2C2 libraries for Illumina and Oxford Nanopore Technologies (ONT) sequencing, respectively.

    Article Title: The cysteinyl leukotriene 3 receptor regulates expansion of IL-25-producing airway brush cells leading to type 2 inflammation.
    Article Snippet: Briefly, Smart-seq2 libraries were sequenced on an Illumina NextSeq500 using a High Output kit to generate 2 × 25 bp reads (plus dual index reads).

    Article Title: Naive stem cell blastocyst model captures human embryo lineage segregation
    Article Snippet: Smart-seq2 libraries were prepared as described ( ) and sequenced using the Illumina Novaseq platform in paired end format.

    Article Title: Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis
    Article Snippet: Smart-seq2 libraries were prepared and sequenced using an Illumina NextSeq500.

    Article Title: A nasal cell atlas reveals heterogeneity of tuft cells and their role in directing olfactory stem cell proliferation
    Article Snippet: Smart-seq2 libraries were sequenced on an Illumina NextSeq500 using a High Output kit to generate 2 x 25 bp reads.

    Gene Expression:

    Article Title: Tuft cell-produced cysteinyl leukotrienes and IL-25 synergistically initiate lung type 2 inflammation.
    Article Snippet: Smart-seq2 libraries were sequenced on an Illumina NextSeq 500 using a High Output kit to generate 2 × 38–base pair reads (plus dual index reads).

    Article Title: Depletion of Hemoglobin Transcripts and Long-Read Sequencing Improves the Transcriptome Annotation of the Polar Bear ( Ursus maritimus )
    Article Snippet: We then sequenced the Smart-seq2 libraries on a multiplexed Illumina HiSeq X 2 × 151 bp run.

    Article Title: Naive stem cell blastocyst model captures human embryo lineage segregation.
    Article Snippet: Smart-seq2 libraries were prepared as described (Picelli et al., 2013) and sequenced using the Illumina Novaseq platform in paired end format.

    Article Title: Generation of an isoform-level transcriptome atlas of macrophage activation
    Article Snippet: Bottom , we extracted RNA and synthesized full-length cDNA which we then processed to generate Smart-seq2 and R2C2 libraries for Illumina and Oxford Nanopore Technologies (ONT) sequencing, respectively.

    Article Title: The cysteinyl leukotriene 3 receptor regulates expansion of IL-25-producing airway brush cells leading to type 2 inflammation.
    Article Snippet: Briefly, Smart-seq2 libraries were sequenced on an Illumina NextSeq500 using a High Output kit to generate 2 × 25 bp reads (plus dual index reads).

    Article Title: Naive stem cell blastocyst model captures human embryo lineage segregation
    Article Snippet: Smart-seq2 libraries were prepared as described ( ) and sequenced using the Illumina Novaseq platform in paired end format.

    Article Title: Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis
    Article Snippet: Smart-seq2 libraries were prepared and sequenced using an Illumina NextSeq500.

    Article Title: A nasal cell atlas reveals heterogeneity of tuft cells and their role in directing olfactory stem cell proliferation
    Article Snippet: Smart-seq2 libraries were sequenced on an Illumina NextSeq500 using a High Output kit to generate 2 x 25 bp reads.



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    Image Search Results


    Increased chromatin accessibility in knockout round spermatids. A).Volcano plot of DEGs between control and Eif5a SKO samples.FDR<0.05. B.The average tag density plot(top pannel) and heatmaps (bottom pannel)around TSS (±3 kb) for the enrichment of ATAC‐seq reads in control and Eif5a SKO round spermatids. C).Plot shows Gain and Loss sites in all control and Eif5a SKO sample replicates. D).Venn diagram showing the overlap of differential peaks identified by CUT&Tag and ATAC‐seq between WT and CKO groups. E).Four‐quadrant scatter plot comparing the log 2 fold changes of significantly differential peaks (FDR < 0.05) from H3K4me3 CUT&Tag (x‐axis) and ATAC‐seq (y‐axis). The Pearson correlation coefficient for the compared data is 0.468. Pearson's *r* = 0.468. F) Venn diagram show shared genes between ATAC‐seq (FDR<0.05) and Smart‐seq2 (FDR<0.05, FC>1.5).

    Journal: Advanced Science

    Article Title: EIF5A Couples Translational Control With Transcriptional Reprogramming Through Chromocenter Reorganization During Spermiogenesis

    doi: 10.1002/advs.202517423

    Figure Lengend Snippet: Increased chromatin accessibility in knockout round spermatids. A).Volcano plot of DEGs between control and Eif5a SKO samples.FDR<0.05. B.The average tag density plot(top pannel) and heatmaps (bottom pannel)around TSS (±3 kb) for the enrichment of ATAC‐seq reads in control and Eif5a SKO round spermatids. C).Plot shows Gain and Loss sites in all control and Eif5a SKO sample replicates. D).Venn diagram showing the overlap of differential peaks identified by CUT&Tag and ATAC‐seq between WT and CKO groups. E).Four‐quadrant scatter plot comparing the log 2 fold changes of significantly differential peaks (FDR < 0.05) from H3K4me3 CUT&Tag (x‐axis) and ATAC‐seq (y‐axis). The Pearson correlation coefficient for the compared data is 0.468. Pearson's *r* = 0.468. F) Venn diagram show shared genes between ATAC‐seq (FDR<0.05) and Smart‐seq2 (FDR<0.05, FC>1.5).

    Article Snippet: The Smart‐seq2 library sequencing was performed by Novogene on Illumina platforms, generating 150 bp paired‐end reads.

    Techniques: Knock-Out, Control

    Proteomic alterations associated with transcriptional changes induced by Eif5a deletion. A).Venn diagram of shared genes between Smart‐seq2 (P value<0.05, FC>1.5) and Proteomics (P<0.05, FC>1.5) analyses. B).GO enrichment analysis based on the 119 commonly upregulated genes. C).QRT‐PCR analysis of candidate genes that were consistently dysregulated in both the transcriptome and proteome of Eif5a SKO testes. Data are presented as mean ± SD from three independent biological replicates (n = 3). Statistical significance was determined using a two‐tailed, unpaired Student's t‐test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). D). Western blots show SPATA1, SPACA3 and SPACA9 proteins in Eif5a SKO and control mice. β‐Actin served as the loading control. E). IGV visualization of genomic regions harboring acrosome‐related ( Spaca3, Ly6K,Spaca9,Spata1,Lamp2 ) and microtubule‐associated ( Ccdc169, Dynlt3 ) genes. Top: ATAC‐seq tracks showing chromatin accessibility in control (blue) versus SKO (red) round spermatids. Bottom: Corresponding Smart‐seq2 coverage.

    Journal: Advanced Science

    Article Title: EIF5A Couples Translational Control With Transcriptional Reprogramming Through Chromocenter Reorganization During Spermiogenesis

    doi: 10.1002/advs.202517423

    Figure Lengend Snippet: Proteomic alterations associated with transcriptional changes induced by Eif5a deletion. A).Venn diagram of shared genes between Smart‐seq2 (P value<0.05, FC>1.5) and Proteomics (P<0.05, FC>1.5) analyses. B).GO enrichment analysis based on the 119 commonly upregulated genes. C).QRT‐PCR analysis of candidate genes that were consistently dysregulated in both the transcriptome and proteome of Eif5a SKO testes. Data are presented as mean ± SD from three independent biological replicates (n = 3). Statistical significance was determined using a two‐tailed, unpaired Student's t‐test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). D). Western blots show SPATA1, SPACA3 and SPACA9 proteins in Eif5a SKO and control mice. β‐Actin served as the loading control. E). IGV visualization of genomic regions harboring acrosome‐related ( Spaca3, Ly6K,Spaca9,Spata1,Lamp2 ) and microtubule‐associated ( Ccdc169, Dynlt3 ) genes. Top: ATAC‐seq tracks showing chromatin accessibility in control (blue) versus SKO (red) round spermatids. Bottom: Corresponding Smart‐seq2 coverage.

    Article Snippet: The Smart‐seq2 library sequencing was performed by Novogene on Illumina platforms, generating 150 bp paired‐end reads.

    Techniques: Quantitative RT-PCR, Two Tailed Test, Western Blot, Control